| Vector |
Construct size (kb): 4.349999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DR1*
Genomic copy number: unique
Gene name: transcription repressor (interacting with the TATA-binding
protein)
Contains complete coding sequence?: Y
Tissue: HeLa cell line
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.4
Cross references: DNA Seq. Acc.: M97388 Insert lengths(kb): 1.399999976158142 Tissue: HeLa cell line Gene product: transcription repressor (interacting with the TATA-binding protein) [DR1*] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.9, 0.74; KpnI--3.3, 1.4; SstI--4.6; PvuII--2.6, 0.9, 0.72, 0.58; XbaI--3.2, 0.94, 0.58; BamHI/HindIII--2.9, 1.70. The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.68; KpnI--0.14, 0.15; SmaI--0.44; PvuII--0.40, 1.09; XbaI--0.32, 1.2. Detects transcripts of 3.4 and 1.5 kb. Transcription from the T7 promoter gives the sense strand. |
| References |
Inostroza JA, et al. Drl, a TATA-binding protein-associated phosphoprotein and inhibitor of class II gene transcription. Cell 70: 477-489, 1992. PubMed: 1339312
Juan A Inostroza, personal communication
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