(BamHI-290-) HindIII-1050- XbaI-875- HindIII-100- AvaI-125- XhoI-570- XbaI-190- HindIII-100- XbaI-100- HindIII-190- EcoRI. The PstI, KpnI and BamHI sites are unique within the insert. Insert contains the following sites separated by (bp)(approx): EcoRI-475- XbaI-140- HindIII-1050- HindIII-170- EcoRV-50- NcoI-70- AvaI-100- XhoI-190- PstI-570- HindIII-190- NcoI-190- HindIII-620- EcoRV-100- AvaI-50- KpnI-30- EcoRV-290- BamHI-290-. Restriction digests of the clone give the following sizes (kb): PstI--9.4, 2.4; BamHI--6.8, 5.1; EcoRI--8.6, 3.0. Distribution and titer of virion DNA varied greatly between leaflets from the same infected plant. Non-infectious full-length clone of strawberry vein banding virus useful in diagnosing the infection. Isolated from Fragaria vesca. Purified virion DNA digested with EcoRI and cloned into EcoRI site of pUC8. Digested recombinants that co-migrated with full-length linear virion DNA were further screened by Southern blot. |
