| 產品名稱 |
pRSV-hjD |
| 商品貨號 |
B172529 |
| Designations |
pRSV-hjD |
| GenBank Number |
X56681
|
| Species |
Homo sapiens, human |
| Depositors |
Y Shaul |
| Applications |
produces protein jun D proto-oncogene |
| Vector |
Construct size (kb): 7.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: RSV-TG
Intact vector size: 4.500
Type of vector: plasmid
Vector end: BamHI
Vector end: SmaI
Cloning sites: BamHI HindIII
Polylinker sites:
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
promoter for expression: RSV LTR, ->
restriction site: HindIII, XbaI, BamHI, ->
restriction site: SmaI, BglII, SalI, KpnI, ->
transcription terminator: SV40 polyadenylation, ->
marker(s): ampR
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: JUND
Gene name: jun D proto-oncogene
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 p13.1
Type of DNA: genomic
Insert 5' end: BamHI
Insert 3' end: DraI
Insert size (kb): 2.5
Cross references: DNA Seq. Acc.: X56681
Nucleotides 801-2500 of the insert correspond to
nucleotides 1-1700 of X56681. Insert lengths(kb): 2.5 Gene product: jun D proto-oncogene [JUND] Target Gene: jun D proto-oncogene |
| Insert Size (kb) |
2.5 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/BglII--3.5, 2.5, 0.85, 0.35; BamHI--3.5, 3.5; BglII--6.6, 0.3; XhoI--6.9; KpnI--6.9. The insert contains the following restriction sites (approximate kb from the 5' end): NotI--0.87, 1.53, 2.27; RsaI--2.01, 2.32; XhoI--1.28. |
| References |
Berger I, Shaul Y. Structure and funcation of human jun-D. Oncogene 6: 561-566, 1991. PubMed: 1903194
Faktor O, Shaul Y. The identification of hepatitis B virus X gene responsive elements reveals functional similarity of X and HTLV-I tax. Oncogene 5: 867-872, 1990. PubMed: 2359621
|
