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Size (kb): 4.5000000000000000

Vector: pLinkCAT (plasmid)

Promoters: Promoter none

Construction: pSV2cat, polylinker

Marker(s):ampR

Construct size (kb): 4.5

Features: insert detection: CAT
marker(s): ampR
promoter: none
replicon: pMB1
terminator: none
enhancer: none

promoter-cloning vector

shuttle vector

Restriction digests of the clone give the following sizes (kb): HindIII--4.5; BglII--4.5; SstI--4.5; consistent with deposited DNA.

This is a promoter-cloning shuttle plasmid vector with a polylinker which can facilitate both molecular cloning of a DNA fragment as well as controlled deletion of sequence from either end of the insert.

This was constructed from the large AccI/HindIII pSV2CAT fragment and a polylinker with AccI and HindIII ends such that the AccI at the end was lost.

Temperature: 37.0°C