Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 1.35; EcoRI/BamHI--2.7, 1.35; EcoRI--4.0; HindIII--4.0. The SacI site is missing. RFLPs detected at normal hybridization stringency; washes at 0.1X SSC, 0.1% SDS, 60 C; probe isolated from cosmid cRMU1. Enzyme(s) not detecting polymorphism: PstI, HindIII. |