Restriction digests of the clone give the following sizes (kb): XbaI/HindIII--2.95, 1.0; PstI--3.2, 0.75; BglI--2.1, 1.3, 0.6; PvuII--2.55, 0.9, 0.4; BamHI--3.2, 0.75. A partial cDNA clone that corresponds to nt 601-1585 of the published sequence (coding region of nt 413-1582). Transcription from the T7 promoter gives the sense strand. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.28; BglI--0.38; PvuII--0.7, 0.98; BamHI--0.74; SmaI--0.85. |
