| 產品名稱 |
pRS405 |
| 商品貨號 |
B173125 |
| Designations |
pRS405 |
| Depositors |
P Hieter |
| Biosafety Level |
1 |
| Host |
Shipped in: E. coli DH5alpha |
| Vector Information |
Size (kb): 5.507 Vector: pRS405 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pBluescriptII KS+ Marker(s):LEU2,ampR Construct size (kb): 5.507 Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: f1
replicon: pMB1
MCS: KpnI...SacI |
| Applications |
YI-type (integrating) shuttle vector |
| Comments |
Restriction digests of the clone give the following sizes (kb): ClaI--3.95, 1.55; BglI--3.8, 1.7; PvuII--5.0, 0.45. A YI-type shuttle vector with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436
Christianson TW, et al. Multifunctional yeast high-copy-number shuttle vectors. Gene 110: 119-122, 1992. PubMed: 1544568
|
| Related Products |
component of:ATCC 87533 component of:ATCC 87536 |
| Shipped |
frozen |
| Shipping Information |
?Freeze dried phagemid in E. coli |
