| Insert |
DNA: genomic Insert lengths(kb): 7.0 Gene product: DNA segment, Chr 16, human D21S16 [D21S16] Alleles: s, A1, B1, B2, A2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--7.2, 2.8; HindIII--5.0, 4.2, 0.92; BamHI--8.4, 1.6; PstI--3.5 (doublet), 3.1; SmaI--10.0. Cross-hybridizes to mouse sequences and detects an RFLP in mice: using XbaI--M spretus, 8.2 kb; C57BL/6J, 3.7 kb. Isolated from the lambdagtWES.lambdaB library of Krumlauf et al. Enzyme(s) not detecting polymorphism: BglII, EcoRI, HindIII, MspI, PstI, PvuII, SstI, TaqI, XmnI. |
| References |
Cheng SV, et al. Comparative mapping of DNA markers from the familial Alzheimer disease and Down syndrome regions of human chromosome 21 to mouse chromosomes 16 and 17. Proc. Natl. Acad. Sci. USA 85: 6032-6036, 1988. PubMed: 2901095
Reeves RH, et al. Sex, strain, and species differences affect recombination across an evolutionary conserved segment of mouse chromosome 16. Genomics 8: 141-148, 1990. PubMed: 1981993
Stinissen P, et al. The pericentromeric 21 DNA marker pGSM21 (D21S13) contains an expressed HTF island. Genomics 7: 119-122, 1990. PubMed: 1692297
Stewart GD, et al. Cloned DNA probes regionally mapped to human chromosome 21 and their use in determining the origin of nondisjunction. Nucleic Acids Res. 13: 4125-4132, 1985. PubMed: 3839305
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