-20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Applications
DNA Quality Control Information
High molecular weight DNA is appropriate for polymerase chain reaction (PCR)* process and other molecular biology applications.
Comments
DNA Quality Control Information
>100 kb in size by agarose gel electrophoresis
No RNA detected by agarose gel electrophoresis
A260/A280 ratio >1.8
Tested and verified for PCR amplification and restriction enzyme digestion
High molecular weight DNA is appropriate for polymerase chain reaction (PCR)* process and other molecular biology applications. *The PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.
High molecular weight DNA isolated from the HCC2157 BL EBV-transformed lymphoblastoid cell line.
Formulation: 1X TE buffer (10 mM Tris-HCl, 0.1 mM EDTA, pH 8.0)
-20C or lower
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Introduction
Cell Line Description:
HCC2157 BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV)-transformation of peripheral blood lymphocytes obtained from the same patient as HCC2157.? HCC2157 was initiated from a 48 year old Black female with primary breast ductal carcinoma, and the DNA is available as ATCC CRL-2340D.
Quality Control Specifications
> 100 kb in size by agarose gel electrophoresis
No RNA detected in the agarose gel
A260/A280 ratio ≥ 1.8
Tested and verified for PCR amplification and restriction digestion.
