Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Donor host for conjugative transfer of oriT-containing plasmids that utilize the R6Kgamma origin of replication. Also useful for transposon mutagenesis.
Phenotypes verified: Tn7 lac+ recA pir+ RP4tra+.
Medium
ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Name of Depositor
BL Wanner
Special Collection
NCRR Contract
References
Metcalf WW, et al. Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers. Gene 138: 1-7, 1994. PubMed: 8125283
