| Vector |
Construct size (kb): 6.300000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD36
Gene name: CD36 antigen (collagen type I receptor, thrombospondin
receptor)
Contains complete coding sequence?: Y
Chromosome: 7; Localization: 7 q11.2
Tissue: erythroid leukemia (HEL) cell line
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.8
Cross references: DNA Seq. Acc.: L06850
Nucleotides 1-1820 of the insert correspond to
nucleotides 1-1820 of L06850. Insert lengths(kb): 1.799999952316284 Tissue: erythroid leukemia (HEL) cell line Gene product: CD36 antigen (collagen type I receptor, thrombospondin receptor) [CD36] |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--3.05, 1.85, 1.25; EcoRI--2.55, 1.85 (doublet); PstI--2.9, 1.1, 0.67, 0.60, 0.32; PvuII--2.25, 1.85, 1.5, 0.8; XbaI--4.0, 1.85, 0.30. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.68; NdeI--1.10; PstI--0.47, 0.75, 1.29; PvuII--0.56; SphI--1.52; XbaI--1.65. The insert is an alternatively spliced product containing exon 2b in the 5' untranslated region. Contains two inserts, tandemly repeated. Oriented with the 5' end closer to the HindIII site of the polylinker. |
| References |
Taylor KT, et al. Characterization of two alternatively spliced 5'-untranslated exons of the human CD36 gene in different cell types. Gene 133: 205-212, 1993. PubMed: 7693552
Kirk T Taylor, personal communication
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