Quantitative Synthetic Cyclospora cayetanensis DNA
商品貨號
B173332
Strain Designations
Quantitative Synthetic Cyclospora cayetanensis DNA
Application
ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine protozoan load and for other molecular biology applications.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen Specification range: 1 x 105 to 1 x 106 copies/μL 100 μL per vial in Biomatrica DNAstable
Storage Conditions
-20°C or colder
Intended Use
This product is intended for research use only. It is not intended for any animal or human therapeutic or diagnostic use. The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.
Comments
Manufactured under ISO 13485 guidance
Preparation includes the full 18S rRNA gene sequence, and full ITS1 and ITS2 sequences.
The following primers and probe can be used with this nucleic acid preparation RefVarma M, et al. Detection of Cyclospora cayetanens using a quantitative real-time PCR assay. J. Microbiol. Methods 53(1): 27-36, 2003. PubMed: 12609720:
Forward primer: TGAACTCATTGGACTGACCAGC
Reverse primer: ACTTTTGCATCCTTTAGAGGGCT
Probe: FAM-CAACTTTCCGACCAGCTCCGCGAA-BHQ1
Name of Depositor
ATCC
Special Collection
DNA
References
Varma M, et al. Detection of Cyclospora cayetanens using a quantitative real-time PCR assay. J. Microbiol. Methods 53(1): 27-36, 2003. PubMed: 12609720