| Vector |
Construct size (kb): 5.230000019073486 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PFN*
Gene name: profilin II
Contains complete coding sequence?: U
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 2.0
Cross references: DNA Seq. Acc.: L10678; EST: EST06533
Nucleotides 13-291 of the insert correspond to
nucleotides 150-428 of L10678 with 88% identity.
DNA Seq. Acc.: L10678; EST: EST06532
Nucleotides 1-187 of the insert correspond to
nucleotides 1693-1507 of L10678 with 98% identity.
DNA Seq. Acc.: T08640; EST: EST06532; Primer: M13-21; THC
id: THC125637
DNA Seq. Acc.: T08641; EST: EST06533; Primer: M13 Reverse;
THC id: THC104389 Insert lengths(kb): 2.0 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: profilin 2 [PFN*] |
