| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--17.0, 9.8, 7.6, 7.0, 4.2, 0.58; EcoRI--20.0, 11.0, 5.4, 2.3, 1.6, 1.5, 1.2, 1.0, 0.87, 0.66; HindIII--24.0, 6.6 (doublet), 2.3; XbaI--21.0, 13.0, 6.0, 3.9, 2.7; KpnI--21.0, 15.0, 4.4, 2.0, 1.5 (doublet); SstI--22.0, 13.0, 6.6, 4.0, 1.9. The MspI polymorphisms have a combined PIC value of 0.69 and a combined heterozygosity of 74%. Enzyme(s) not detecting polymorphism: TaqI, RsaI, BglII, HindIII, HincII, PstI, HaeIII (panel of 5 individuals). |
| References |
Schumm JW, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am. J. Hum. Genet. 42: 143-159, 1988. PubMed: 2892396
Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638
|
