Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
Growth rate markedly stimulated by increases in incubation temperature. Optimum temperature for growth in CE is 39°C. Previously classified as C. psittaci; see Fukushi & Hirai (IJSB. 42: 306, 1992) for pecorum classification.
FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.
Effect on Host
In TC, requires FA to visualize intracellular inclusion bodies. Lethal for CE with hyperemia and hemorrhage in y.s. and embryo
Recommended Host
McCoy (ATCC CRL-1696)
Growth Conditions
Temperature: 37°C Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent as the cylcoheximide will prevent further growth.
Incubation: 48 to 72 hours, a 5%CO2 in air atmosphere is recommended.
Name of Depositor
LA Page
Source
Calf brain from herd in Iowa, USA
References
Fukushi H, Hirai K. Proposal of Chlamydia pecorum sp. nov. for Chlamydia strains derived from ruminants. Int. J. Syst. Bacteriol. 42: 306-308, 1992. PubMed: 1581191
McNutt SH. . Cornell Vet. 30: 437, 1940.
Cross References
Nucleotide (GenBank) :
AJ012173
Chlamydophila pecorum partial rnpB gene for RNase P RNA subunit.
