| 產品名稱 |
pGX28L |
| 商品貨號 |
B173448 |
| Designations |
pGX28L |
| Species |
Pseudopleuronectes americanus, winter flounder |
| Depositors |
WE Moore, T Caceci, WE Moore |
| Applications |
in another host, produces protein artificial antifreeze peptide WF8R |
| Vector |
Construct size (kb): 10.0 |
| Insert |
DNA: Synthetic Insert lengths(kb): 0.3100000023841858 Gene product: artificial antifreeze peptide WF8R [WF8R] Target Gene: artificial antifreeze peptide WF8R |
| Insert Size (kb) |
0.310 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Shipped: ?Freeze dried phagemid in E. coli |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--6.2, 3.4, 0.4; BamHI--6.4, 3.7; EcoRI--10.0; HindIII--10.0. The sequence is a gene for an extended-length structural mimic of the IIC10 peptide from the winter flounder (Pseudopleuronectes americanus) fused to the 8th codon of LacZ'. When the clone is digested with BamHI/EcoRI, the 0.3 kb band is the synthetic antifreeze peptide. For expression of the insert, the plasmid must be transferred to a host expressing T7 RNA polymerase such as JM109(DE3). |
| References |
Thomas Caceci, personal communication
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
