Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; BamHI/PvuII--4.9, 0.5. Shuttle expression vector. Permits expression in both S. cerevisiae and mouse cells. The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter. Constructed by deleting the HpaI (1686)/SmaI (2349) fragment from pCMY0 (ATCC 37820), removing part of the SV40 terminator region from pSV2-cat and part of the URA3-associated sequence from pUT322. |
