| 產品名稱 |
pEGsph50-2 [NEB808] |
| 商品貨號 |
B173684 |
| Designations |
pEGsph50-2 [NEB808] |
| Species |
Neisseria lactamica Hollis et al. |
| Depositors |
New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Applications |
produces protein modification methylase NlaIII produces protein restriction endonuclease SphI |
| Vector |
Construct size (kb): 9.699999809265137 |
| Insert |
DNA: genomic Insert lengths(kb): 1.0 Gene product: modification methylase NlaIII [sphIR] Target Gene: modification methylase NlaIII, restriction endonuclease SphI |
| Insert Size (kb) |
1.0 |
| Media |
ATCC Medium 1065 (see below) plus ampicillin (50.0 mcg/ml) plus kanamycin (20.0 mcg/ml)
ATCC Medium 1065:
Tryptone (Difco 0123), 10.0 g
Yeast Extract (Difco 0127), 5.0 g
NaCl, 10.0 g
Distilled water, 1.0 L
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of p(SYX20)NlaIIIM+ give the following sizes (kb): BamHI--8.8; BamHI/SalI--8.0, 1.10; HindII--4.6, 4.2; BspHI/HindIII--4.6, 4.2. Restriction digests of pEGsph50-2 give the following sizes (kb): BamHI--uncut; BamHI/SalI--8.0; HindIII--7.6; BspHI/HindIII--7.4. Expression of the NlaIII methylase protects the host DNA from digestion with by the SphI nuclease. The methylase is expressed constituitively from the tetR promoter. |
| References |
Guthrie EP. Isolated DNA encoding the SphI restriction endonuclease and related methods for producing the same. US Patent 5,262,318 dated Nov 16 1993
Xu SY, Nwankwo DO. Method for cloning and producing the AatII and AluI restriction endonuclease and methylase and related method for overexpressing restriction endonuclases. US Patent 5,405,768 dated Apr 11 1995
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
