| 產品名稱 |
pGL2BIFN |
| 商品貨號 |
B173701 |
| Designations |
pGL2BIFN |
| Species |
Homo sapiens, human |
| Depositors |
Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V. |
| Applications |
in another host, produces protein interferon, alpha 2 |
| Vector |
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGL2B
Intact vector size: 6.600
Type of vector: plasmid
Cloning sites: EcoRV, BglII
Polylinker sites:
Other unique sites: BamHI, HindIII
Construction: pUC12
Host range: Escherichia coli; filamentous fungi
Features (with orientation and position when available):
marker(s): ampR
replicon: pMB1
promoter: glucoamylase, ->
signal peptide coding region: glucoamylase, ->
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: IFNA2
Genomic copy number: unique
Gene name: interferon, alpha 2
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 p22
Type of DNA: cDNA
Insert 5' end: DdeI Modification: filled
Insert 3' end: BamHI Modification: filled
Insert size (kb):
Cross references: Gene product: interferon, alpha 2 [IFNA2] Target Gene: interferon, alpha 2 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.9, 1.9, 1.2; BamHI--8.0. The construct has been demonstrated to direct secretion of protein after starch induction in both A. niger and A. nidulans. Should be cotransformed with a plasmid expressing argB for selection. |
| References |
Gwynne DI, et al. Vectors in use in filamentous fungi. US Patent 5,198,345 dated Mar 30 1993
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
