| Comments |
XbaI (insert) and HindIII(vector) ends filled in and blunt-end ligated so insert can be removed by XbaI digest. Strongest hybridization to fragments of 680, 1020, 850, and 1870 bp generated by XbaI-cut DNA. Sequence of pE1 differs from pF5 by 15 nucleotides. Hybridizes with DNA from chimpanzee, gorilla, weakly with orangutan, but not with gibbon, Old or New World monkeys. This hybridizes to certain fragments in HinfI and HindIII-digested DNA, but little or no repeat sequence homology is seen with EcoRI, KpnI or PstI-digested DNA. The XbaI repeat is composed of 4 tandem repeats of 171, 170, 171, 170 bp. pF5 hybridizes with all centromeric regions but Y. There is 64% hybridization signal at centromeres, 43% total label on D and G group chromosomes, and no hybridization detected at telomeres. |
