Restriction digests of the clone give the following sizes (kb): EcoRI--12.6; HindIII--12.6; SphI--9.0, 3.6. A shuttle vector permitting autoregulated high-level expression in all gram-negative bacteria. Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids. Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013 (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria. Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation. Differs from pJAK16 (ATCC 77291) only in the orientation of the polylinker. |
