| 產(chǎn)品名稱 |
pGSRK-1 |
| 商品貨號 |
B173808 |
| Designations |
pGSRK-1 |
| Species |
Rattus norvegicus, rat |
| Depositors |
RE Miller |
| Applications |
This clone has been used to isolate mouse genomic glutamine synthetase clones. |
| Vector |
Construct size (kb): 6.0
Name of clone: pGSRK-1
Gene symbol: GLUL
Cloned from: rat kidney
Name of vector: pBR322
Insert site(s): PstI
Insert ends: GC tails
Source of insert DNA: cDNA
Markers: tetR |
| Insert |
DNA: cDNA
Insert lengths(kb): 1.60
Tissue: kidney
Gene product: glutamate-ammonia ligase (glutamine synthase)(glutamine synthetase, glutamate-ammonia ligase, glutamine synthase) [GLUL] |
| Insert Size (kb) |
1.60 |
| Media |
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed in Escherichia coli HB101 containing plasmid |
| Comments |
The PstI site distal to the vector EcoRI site was lost during cloning. The insert contains BamHI (2), EcoRI, EcoRV, and PvuII sites.
-Biochem. Biophys. Res. Commun. 134: 146-151, 1986
Restriction digests of the clone give the following sizes (kb): PstI--6.0; BamHI--3.8, 2.25; EcoRI--4.4, 2.1; HindIII--6.0; EcoRV--4.2, 2.5.
-ATCC staff
This clone has been used to isolate mouse genomic glutamine synthetase clones. It detects mRNAs of about 3.2 kb and 1.6 kb inHTML mouse 3T3-L1 cells and rat liver cells.
-Mol. Cell. Endocrinol. 47: 49-57, 1986 |
| References |
Bhandari B, et al. Cloning, nucleotide sequence, and potential regulatory elements of the glutamine synthetase gene from murine 3T3-L1 adipocytes. Proc. Natl. Acad. Sci. USA 85: 5789-5793, 1988. PubMed: 2901089
Burns DM, et al. Selection of a rat glutamine synthetase cDNA clone. Biochem. Biophys. Res. Commun. 134: 146-151, 1986. PubMed: 2418824
Bhandari B, et al. Glutamine synthetase mRNA in cultured 3T3-L1 adipocytes: complexity, content and hormonal regulation. Mol. Cell. Endocrinol. 47: 49-57, 1986. PubMed: 2875005
|
