Restriction digests of the clone give the following sizes (kb): HindIII--2.9; BamHI--2.9; NcoI--2.9. The NcoI site (CCATGG) includes the initiator methionine codon. The NcoI cloning site can also be used in conjunction with the downstream BamHI site. Differs from pT7/NdeI (ATCC 87050) only in the cloning site and reading frame for the insert. Expression vector, maintained at high copy number. The order of the major features of the plasmid is: SP6 - gene 10 RBS - T7 - NcoI - BamHI - pMB1 ori - ampR. |
