| 產(chǎn)品名稱 |
Influenza A virus (H1N1) |
| 商品貨號(hào) |
B174064 |
| Classification |
Orthomyxoviridae, Influenzavirus A |
| Agent |
Influenza A virus (H1N1) |
| Strain |
A/Swine/1976/31 (TC-adapted) |
| Applications |
Emerging infectious disease research Respiratory research Influenza Research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Derived by adaptation of egg-passage A/Swine/1976/31 (ATCC VR-99) to MDCK (ATCC CCL-34) cells. (Note: ATCC VR-99 and ATCC VR-1682 have not been compared with respect to sequence or infectivity in chick embryo and tissue culture). |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
Derived by adaptation of egg-passage A/Swine/1976/31 (ATCC VR-99) to MDCK (ATCC CCL-34) cells. (Note: ATCC VR-99 and ATCC VR-1682 have not been compared with respect to sequence or infectivity in chick embryo and tissue culture). |
| Effect on Host |
CPE (cell rounding, degeneration, sloughing) CPE (cell rounding, degeneration, sloughing) |
| Growth Medium |
ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
|
| Recommended Host |
Production Host: MDCK (ATCC CCL-34) Alternate Hosts: Chicken embryo; MDCK and monkey kidney cells (MkK) Recommended Host:? MDCK (ATCC? CCL-34?) |
| Growth Conditions |
For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluent (not 100% confluent) using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 35.0°C in a humidified, 5% CO2 atmosphere for 2-4 days, until CPE are well advanced in 100% of the culture. In TC, ATCC VR-1682 should be grown in serum free media that contains 1 ug/ml TPCK-treated trypsin, 0.125% BSA and 1% HEPES (1 molar stock). |
| Name of Depositor |
M.T. Coleman and ATCC |
| Source |
Hog in Iowa, 1931. |
| Year of Origin |
1976 |
| References |
Jensen KE, Peterson WD Jr.. Comparative measurements of antigenic differences among human and swine influenza viruses. J. Immunol. 78: 365-372, 1957. PubMed: 13449325
|
