Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1.0 mL per vial
Storage Conditions
Vapor phase of liquid nitrogen
Comments
Cells contain a GALV plasmid (pGEM13386) and are not infected with a primary infectious GALV. Cells are not G418 resistant. Cells should be monitored by RT-PCR to confirm that viral genes are expressed. At the time of deposit, this line was considered important as a source of early passage GALV SEATO strain, which was considered a reference material of utility to PG13 gene therapy researchers.
Effect on Host
No CPE, RT-PCR to assess for virus presence
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Thaw the cells in a 37°C water bath until the ice is just melted, then add 0.5 mL of cells directly to a T-25 flask containing fresh medium. Post-freeze recovery is slow. Perform complete media renewal on Day 3 and continue media renewal every 3 - 4 days. Monitor the cells for adherence and expect populations of cells to project epithelial structures on approximately Day 4 - Day 8. Cell growth rate significantly increases with subsequent subcultures.
Incubation: 3-5 days, until confluent and passaging or harvesting is needed
Mink cells expressing Gibbon ape leukemia virus (GALV). These infected cells were produced by transfecting a clone of GALV SEATO strain into mink cells and testing cell supernatant after several passages for a sustained peak in reverse transcriptase activity. The clone was derived from sequences obtained from GALV San Francisco strain and SEATO strain.
References
also affects subhuman primates and monkey cells
Mink cells expressing Gibbon Ape Leukemia Virus. These infected cells were produced by transfecting a biologically clone of GALV-SEATO into mink cells and testing cell supernatant after several passages for a sustained peak in reverse transcriptase activity-designating that the cells are productively infected. The GALV clone was derived from sequences obtained from GALV strain San Francisco and SEATO, pGEM-13-386, GaLV-SEATO.
