Insert contains the following restriction sites (in bp from the 5' end): BanII--295; EcoRI--390; HindIII--538; ScaI--159. Restriction digests of the clone give the following sizes (kb): EcoRI--3.7; HindIII--2.7, 1.0; BamHI--2.9, 0.8. Contains a complete coding sequence plus 5' and 3' untranslated regions. The 5' end lacks the first 10 nucleotides. At the amino acid level, the sequence is 92% conserved between bovine and mouse. At the nucleotide level, the sequence is 89% conserved between bovine and mouse. |
