| Comments |
The insert contains the following restriction sites (approximate kb from the 5' end): PvuII--0.01, 0.25, 0.78; KpnI--0.65; PstI--0.68, 0.99. Restriction digests of the clone give the following sizes (kb): HindIII--5.5; PstI--4.4, 0.8, 0.3; NotI--5.5; XbaI/HindIII--4.1, 1.4; XbaI--4.1, 1.4. Does not express the protein when transfected into COS cells. The orientation 3' - 5' relative to the promoter. |
| References |
Cho FS, et al. Cloning of the rat cyclin-dependent kinase 4 cDNA: implication in proliferation-dependent expression in rat tissues. Biochem. Biophys. Res. Commun. 191: 860-865, 1993. PubMed: 8466525
Tim Weaver, personal communication
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