| Vector |
Construct size (kb): 5.71999979019165 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSPORT1
Intact vector size: 4.109
Type of vector: plasmid
Vector end: SalI
Vector end: NotI
Cloning sites: AatII SphI mLuI SunI SnaBI HindIII BamHI XbaI XmaIII NotI
SpeI SstI SalI AccI SmaI EcoRI Kpn2I RsrII PinAI KpnI
Sse8387I PstI
Polylinker sites: AatII SphI mLuI SunI SnaBI HindIII BamHI XbaI XmaIII NotI
SpeI SstI SalI AccI SmaI EcoRI Kpn2I RsrII PinAI KpnI
Sse8387I PstI
Host range: Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: SP6, ->, 164-180
MCS: AatII...PstI, ->, 191-285
promoter for in vitro transcription: T7, <-, 298-318
insert detection: lacZ', <-, 1-341
repressor gene: lacI, <-, 464-1546
replicon: pMB1, 1903-1903
marker(s): ampR, <-, 2661-3521
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: KIR
Other gene symbols: NKB1
Gene name: killer cell inhibitory receptor
ORF's seq. position: GDB probe: GDB:698358 ()
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 q13.4; 19, 374.860000 cR
Tissue: NK3.3 cell line
Type of DNA: cDNA
Insert 5' end: Modification: SalI linker
Insert 3' end: Modification: NotI linker
Insert size (kb): 1.61
Cross references: DNA Seq. Acc.: U24074
Nucleotides 1-1579 of the insert correspond to
nucleotides 1-1579 of U24074. Insert lengths(kb): 1.610000014305115 Tissue: NK3.3 cell line Gene product: killer cell inhibitory receptor [KIR] |
| Comments |
Restriction digests of the clone give the following sizes (kb): NotI/SalI--4.4, 1.6; EcoRV--4.5, 1.5; PstI--5.0, 0.4, 0.35, 0.1; PvuII--4.0, 1.4, 0.6; XhoI--6.0. The insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--1.01; NdeI--0.93; PstI--0.07, 0.46, 0.54, 0.87; PvuII--1.15; SacI--0.48; StuI--1.08; XhoI--0.65. Encodes a variant recognized by monoclonal antibody GL183 but not EB6. |
