| 產(chǎn)品名稱 |
pT7L-21 |
| 商品貨號(hào) |
B174887 |
| Designations |
pT7L-21 |
| Species |
Tetrahymena thermophila Nanney and McCoy |
| Depositors |
Univ. Colorado, TR Cech, Univ. Colorado |
| Applications |
After linearizing with ScaI, the T7 runoff transcript can be used to produce the ribozyme. |
| Vector |
Construct size (kb): 3.200000047683716 |
| Insert |
DNA: genomic Insert lengths(kb): 0.5 Gene product: ribozyme having endoribonuclease activity toward recognition sequence CUCU (RNA, ribosomal) |
| Insert Size (kb) |
0.5 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 0.5; EcoRI--3.2; HindIII--3.2. Distributed in aliquots of 200 ng dried. Constructed by inserting the SphI/HindIII fragment [intervening sequence (IVS) minus the first 44 bp] from pBGST7 (ATCC 40288) into pUC18, and adding a synthetic EcoRI/SphI fragment containing a T7 promoter and bp 22-44 of the IVS. After linearizing with ScaI, the T7 runoff transcript can be used to produce the ribozyme. |
| Classification |
KINGDOM: Protozoa |
| References |
Cech TR, et al. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods. US Patent 4,987,071 dated Jan 22 1991
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
