Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Storage Conditions
Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Preceptrol®
no
Genotype
F- delta(lacIPOZY)X74 galE galK thi rpsL deltaphoA
Type Strain
no
Comments
Host strain (ara+) for use with the pBAD series of expression vectors (ATCC 87393 - 87402).
Metabolism of arabinose results in very rapid repression of expression for pBAD vectors.
Induction of expression in pBAD vectors can be maintained at high levels. Induction response is not linear with respect to inducer (arabinose) concentrations.
Medium
ATCC® Medium 1445: Nutrient agar or broth with 20 mcg/ml streptomycin
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor
L Guzman, J Beckwith
Special Collection
NCRR Contract
References
Strauch KL, Beckwith J. An Escherichia coli mutation preventing degradation of abnormal periplasmic proteins. Proc. Natl. Acad. Sci. USA 85: 1576-1580, 1988. PubMed: 3278319
Guzman LM, et al. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177: 4121-4130, 1995. PubMed: 7608087
