Restriction digests of the clone give the following sizes (kb): BamHI--12.0, 1.8; EcoRI--6.2, 4.4, 2.4, 0.8; SalI--14.0. LEU2-d is a promoter-defective allele of the yeast LEU2 gene, which increases plasmid copy number to 200-300 copies in the presence of LEU2 selection (host grown in medium lacking leucine). YE-type shuttle vector. The order of the major features in this plasmid is: LEU2-d - ori(2 micron) - HIS3 - SalI site - ampR. |
