| Insert |
DNA: genomic Insert lengths(kb): 19.0 Tissue: liver Gene product: DNA Segment, single copy [D7S54] Alleles: A5, A6, B1, B2, B4, A1, A3, A4, B3, B5, A2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII-->23.0, 15.0, 2.9 (doublet); KpnI--21.0, 15.0, 9.4, 3.3, 1.4; SacI--23.0, 14.0, 4.2, 4.0, 1.9, 1.1; SmaI--23.0, 9.0, 7.0, 3.5, 2.6, 1.3, 0.6; EcoRI--20.0, 11.0, 9.4, 4.2, 3.7, 1.5, 0.4. The MspI and TaqI polymorphisms have a combined PIC value of 0.71 and a combined heterozygosity of 79%. Enzyme(s) not detecting polymorphism: BglII, BamHI (panel of 5 individuals). |
| References |
Schumm JW, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am. J. Hum. Genet. 42: 143-159, 1988. PubMed: 2892396
Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638
Kazazian HH Jr., et al. Characterization of a spontaneous mutation to a B-thalassemia allele. Am. J. Hum. Genet. 38: 860-867, 1986. PubMed: 3014870
Barker D, et al. Genetic linkage map of human chromosome 7 with 63 DNA markers. Proc. Natl. Acad. Sci. USA 84: 8006-8010, 1987. PubMed: 2891136
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