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Trypanosoma lewisi (Kent) Laveran and Mesnil
Trypanosoma lewisi (Kent) Laveran and Mesnil
規格:
貨期:
編號:B175402
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Trypanosoma lewisi (Kent) Laveran and Mesnil
商品貨號 B175402
Strain Designations New Orleans-67
Application
Vector borne research
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
rat, Rattus norvegicus, New Orleans, 1967
Product Format frozen
Type Strain no
Comments
Flow cytometric analysis of immune-globulin and complement component C3 on the surface
Sensitivity to alternate complement pathway-mediated lysis
use of plastic ampoules for freeze preservation
Growth Conditions
Duration: in vivo, laboratory rat
Protocol: ATCCNO: 30085 SPEC: Inject the entire thawed contents of the ampule intraperitoneally into an adult rat known to be free of blood parasites. The strain should be monitored, especially after infecting with thawed material. To infect additional rats, draw a drop of tail blood into a 1.0 ml tuberculin syringe containing 0.5 ml of sterile buffered anticoagulant, mix, and inoculate 0.1-0.2 ml of the suspension intraperitoneally/rat. Only a few trypanosomes are needed to infect a non-immune rat. However, do not wait until the blood is swarming with trypanosomes. The time for peak parasitemia may be within 4-5 days if large inocula are given. Peak parasitemia from the thawed material may be slightly sooner or later.
Subcultivation
Protocol: ATCCNO: 30085 SPEC: Inject the entire thawed contents of the ampule intraperitoneally into an adult rat known to be free of blood parasites. The strain should be monitored, especially after infecting with thawed material. To infect additional rats, draw a drop of tail blood into a 1.0 ml tuberculin syringe containing 0.5 ml of sterile buffered anticoagulant, mix, and inoculate 0.1-0.2 ml of the suspension intraperitoneally/rat. Only a few trypanosomes are needed to infect a non-immune rat. However, do not wait until the blood is swarming with trypanosomes. The time for peak parasitemia may be within 4-5 days if large inocula are given. Peak parasitemia from the thawed material may be slightly sooner or later.
Name of Depositor RG Yaeger
Year of Origin 1967
References

Gannon JT, Linke HA. Growth studies on xenic cultures of Entamoeba gingivalis using established media. Int. J. Parasitol. 19: 835-838, 1989. PubMed: 2635159

Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238

Sturtevant JE, Balber AE. Trypanosoma lewisi: restriction of alternative complement pathway C3/C5 convertase activity. Exp. Parasitol. 63: 260-271, 1987. PubMed: 3556206

Sturtevant JE, Balber AE. Flow cytometric analysis of immunoglobulin and complement component C3 on the surface of Trypanosoma lewisi. J. Protozool. 33: 197-203, 1986. PubMed: 3525820

Sturtevant JE, Balber AE. Externally disposed membrane polypeptides of intact and protease- treated Trypanosoma lewisi correlated with sensitivity to alternate complement pathway-mediated lysis. Infect. Immun. 42: 869-875, 1983. PubMed: 6358042

Cross References

Nucleotide (GenBank) : AJ223566 Trypanosoma lewisi, small subunit ribosomal RNA gene.

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
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李經理 13626845108 972239479
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