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pLOI292
pLOI292
規(guī)格:
貨期:
編號(hào):B175477
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pLOI292
商品貨號(hào) B175477
Designations pLOI292
Species Zymomonas mobilis (Lindner) Kluyver and van Niel
Depositors University of Florida, Gainesville, LO Ingram, University of Florida, Gainesville
Applications
produces protein alcohol dehydrogenase II
produces protein pyruvate decarboxylase
Vector
Construct size (kb): 16.36000061035156
Insert
DNA: genomic
Insert lengths(kb): 3.099999904632568
Gene product: alcohol dehydrogenase II [pdc]
Target Gene: pyruvate decarboxylase, alcohol dehydrogenase II
Insert Size (kb) 3.100
Media 1675 plus glucose (2%)
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--13.4, 3.1; EcoRI--16.5; HindIII--9.8, 3.9, 1.6, 1.0.
The GenBank accession number for the adh insert is M15394.
The vector contains oriV from RSF1010, mob sequences from RSF1010 and pSUP205, and a Zymomonas mobilis promoter upstream of the BamHI cloning site.
Constructed by cloning a blunted EcoRI/SalI fragment containing both pdc and adhB into the SmaI site of pUC19. The BamHI fragment from this construct was cloned into the vector, such that both genes are controlled by the Z. mobilis promoter.
References

Ingram LO, et al. Ethanol production by Escherichia coli strains co-expressing Zymomonas pdc and adh genes. US Patent 5,000,000 dated Mar 19 1991

Conway T, et al. Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis. J. Bacteriol. 169: 2591-2597, 1987. PubMed: 3584063

Conway T, et al. Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase. J. Bacteriol. 169: 949-954, 1987. PubMed: 3029037

Conway T, et al. Expression vector for Zymomonas mobilis. Appl. Environ. Microbiol. 53: 235-241, 1987.

Ingram LO, Conway T. Expression of different levels of ethanologenic enzymes from Zymomonas mobilis in recombinant strains of Escherichia coli. Appl. Environ. Microbiol. 54: 397-404, 1988.

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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