ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): PstI/HindIII--4.3, 1.3; EcoRI--uncut; BamHI--5.0, 0.62.
Constructed by amplification from genomic DNA using the primers: 5'-ATATTCTGCAGACCACTCA-3', generating a PstI site within the natural AUG codon, and 5'-GGCGCAAGCTTTTACCTGCTGGCGTATCGTTGT-3', generating a HindIII site after the stop codon.
Initiation and termination sequences were then ligated to the respective ends.
