| 產品名稱 |
FLHSL |
| 商品貨號 |
B176118 |
| Designations |
FLHSL |
| GenBank Number |
X51415
|
| Species |
Rattus norvegicus, rat |
| Depositors |
MC Schotz |
| Vector |
Construct size (kb): 6.199999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: rat
Gene symbol: LIPE
Genomic copy number: unique
Gene name: lipase, hormone-sensitive
Contains complete coding sequence?: Y
Tissue: adipose
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 3.225
Cross references: DNA Seq. Acc.: X51415
Nucleotides 1-3225 of the insert correspond to
nucleotides 1-3225 of X51415. Insert lengths(kb): 3.224999904632568 Tissue: adipose Gene product: lipase, hormone-sensitive [LIPE] Alleles: S, B, |
| Insert Size (kb) |
3.225 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRV/SmaI--3.2, 3.0; EcoRI--3.0, 2.5, 0.8; BamHI--4.4, 2.1. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--1.16, 1.98; BamHI--2.02; EcoRI--2.49. |
| References |
Holm C, et al. Hormone-sensitive lipase: sequence, expression, and chromosomal localization to 19 cent-q13.3. Science 241: 1503-1506, 1988. PubMed: 3420405
Holm C, et al. Nucleotide sequence of rat adipose hormone sensitive lipase cDNA. Nucleic Acids Res. 16: 9879, 1988. PubMed: 3186461
|
