Gene product: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) [F9]
Alleles: A1, A2, D2, C1, D1, C2
Insert Size (kb)
5.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.21, 4.83; HindIII--7.99, 2.53; BamHI--12.41; PstI--12.11; PvuII--7.99, 3.67.
The insert can be separated after a EcoRI/HhaI digest of the clone. The insert is not cut but the vector is digested to fragments <400 bp.
The XmnI RFLP has demonstrated linkage to X-linked albinism-deafness syndrome (ADFN) at about 8.5 cM.
This probe does not detect the MspI RFLP.
The depositor recommends hybridization at 42C in 40% formamide and washing at 60C, 0.5X SSC, 0.1% SDS.
References
Vincent A, et al. The polymorphic marker DXS304 is within 5 centimorgans of the fragile X locus. Genomics 5: 797-801, 1989. PubMed: 2574147
Shiloh Y, et al. Genetic mapping of X-linked albinism-deafness syndrome (ADFN) to Xq26.3-q27.1. Am. J. Hum. Genet. 47: 20-27, 1990. PubMed: 2349949
Gejman PV, et al. Manic depressive illness not linked to factor IX region in an independent series of pedigrees. Genomics 8: 648-655, 1990. PubMed: 1980485
