| 產品名稱 |
ptac12 |
| 商品貨號 |
B176274 |
| Designations |
ptac12 |
| Depositors |
E Amann |
| Biosafety Level |
1 |
| Vector Information |
Size (kb): 2.60 Vector: ptac12 (plasmid) Promoters: Promoter tac Construction: pEA300 (ATCC 37181), pGL101 Marker(s):ampR Construct size (kb): 2.6 Features: marker(s): ampR
promoter: tac
replicon: pMB1
enhancer: none |
| Applications |
contains easily purifiable cassette(s) for construction expression vector |
| Comments |
A 55 bp HpaII/PvuII fragment containing the -10 region of the lacUV5 promoter was inserted into the ClaI site of pEA300 (ATCC 37181). Contains the lac operator. The PvuII site is located 5 bp downstream of the lacZ ribosome binding sequence. The tac promoter can be excised by a EcoRI/PvuII digest. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: CSHL Press; 1982.
Amann E, et al. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25: 167-178, 1983. PubMed: 6363212
|
| Shipped |
freeze-dried |
| Shipping Information |
Shipped: ?Freeze dried E. coli containing the plasmid. |
