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<i>Scheffersomyces stipitis</i> (Pignal) Kurtzman et Suzuki

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產(chǎn)品名稱	Scheffersomyces stipitis (Pignal) Kurtzman et Suzuki
商品貨號(hào)	B176299
Deposited As	Pichia stipitis Pignal
Strain Designations	CBS 6054 [CCRC 21777, IFO 10063, NRRL Y-11545]
Application	Bioethanol production
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format	freeze-dried
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Type Strain	no
Preceptrol®	no
Genome Sequenced Strain	Yes
Comments	Use of corn steep liquor in fermentation of D-xylose Genome sequencing strain (completed by the Joint Genome Institute at the Department of Energy, USA).
Morphology	On YM medium at 25°C after 2 days, colonies creamy white to dingy white, smooth, slightly raised, margin entire. Cells subglobose to ellipsoid, smooth, guttulate, 4-8 X 3-5 μm.
Medium	ATCC^® Medium 200: YM agar or YM broth ATCC^® Medium 1245: YEPD ATCC^® Medium 28: Emmons' modification of Sabouraud's agar
Growth Conditions	Temperature: 24°C to 26°C Atmosphere: Typical aerobic
Sequenced Data	18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCGCTTAACTGCGCGGCGAAAAAACCTTACACACAGTGTTTTCTTTATTAGAAACTATTGCTTTGGTCTGGCTCAGAAATGAGTTGGGCCAGAGGTTTACCAAACTTCAATTTTATTGAATTGTTATTTTATTAATTTGTCAATTTGTTGATTAAATTCAAAAATCTTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATATGAATTGCAGATTTTCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAAAGGGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAACCCTCGGGTTTGGTATTGAGTGATACTCTTAGTCGAACTAGGCGTTTGCTTGAAAAGTATTGGCACGAGTGGTACTAAATAGTACTGACAGAATATTTCAATGTATTAGGTTTATCCAACTCGTTGAGACTTCTGGCGGTGAATTTTTGGTATATTGGCTTTGCCTTACAAAACAACAAACAAGTTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 26S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGCACCTTCGGTGTCCGAGTTGTAATTTGAAGAAGGTAACTTTGGAGTCAGCTCTTGTCTATGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTGCGATGAGATGTCTGATTCTATGTAAAGTGCTTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGTTTGAGATCAGACTTGGTATTTTGTATGTCTTGCTTTCGGGTGGGGCCTCTACAGTTTACTGGGCCAGCATCGGTTTGGACGGTAGGATAATGACATTGGAATGTGGCACCACTTCGGTGGTGTGTTATAGACTTTGTTGATACTGCCTGTCTAGACCGAGGACTGCGTCTTTGACTAGGATGCTGGCATAATGATCTTAAACCGC
Name of Depositor	CBS
Chain of Custody	ATCC <-- CBS <-- J Santa Maria
Cross References	Nucleotide (GenBank) : KU729088 ITS including 5.8S rRNA gene Nucleotide (GenBank) : KU729191 D1/D2 region of 28S rRNA gene Nucleotide (GenBank) : CM000437 Pichia stipitis CBS 6054 chromosome 1, whole genome shotgun sequence. Nucleotide (GenBank) : AAVQ00000000 Scheffersomyces stipitis CBS 6054 chromosome 1, whole genome shotgun sequencing project Nucleotide (GenBank) : AF030426 nucleotide sequence of PsCYC1 Nucleotide (GenBank) : U08628 Pichia stipitis CBS 6054 ATCC 58785 autonomous replication Nucleotide (GenBank) : U08629 Pichia stipitis CBS 6054 ATCC 58785 orotidine-5'-phosphate Nucleotide (GenBank) : AF127801 Pichia stipitis xylitol dehydrogenase (XYL2) gene, complete cds.
References	Toivola A, et al. Alcoholic fermentation of D-xylose by yeasts. Appl. Environ. Microbiol. 47: 1221-1223, 1984. Deed RW, et al. Xylitol production by recombinant Saccharomyces cerevisiae. Bio-Technology 9: 1090-1095, 1991. PubMed: 1367625 Amartey S, Jeffries TW. Comparison of corn steep liquor with other nutrients in the fermentation of D-Xylose by Pichia stipitis CBS 6054. Biotechnol. Lett. 16: 211-214, 1994. Shi NQ, et al. Disruption of the cytochrome c gene in xylose-utilizing yeast Pichia stipitis leads to higher ethanol production. Yeast 15: 1021-1030, 1999. PubMed: 10455226 Jeffries TW, et al. Genome sequence of the lignocellulose-bioconvertingand xylose-fermenting yeast Pichia stipitis. Nat. Biotech. 25: 319-326, 2007. PubMed: 17334359 Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009. PubMed: 19664262 Rosenblad MA, et al. Kinship in the SRP RNA family. RNA Biol. 6: 508-516, 2009. PubMed: 19838050 Taylor DJ, Bruenn J. The evolution of novel fungal genes from non-retroviral RNA viruses. BMC Biol. 7: 88, 2009. PubMed: 20021636 Andersen ES, et al. The tmRDB and SRPDB resources. Nucleic Acids Res. 34: D163-D168, 2006. PubMed: 16381838 Shi NQ, et al. SHAM-sensitive alternative respiration in the xylose-metabolizing yeast Pichia stipitis. Yeast 19: 1203-1220, 2002. PubMed: 12271457 Jin YS, et al. Molecular cloning of XYL3 (D-xylulokinase) from Pichia stipitis and characterization of its physiological function. Appl. Environ. Microbiol. 68: 1232-1239, 2002. PubMed: 11872473 Shi NQ, et al. Characterization and complementation of a Pichia stipitis mutant unable to grow on D-xylose or L-arabinose. Appl. Biochem. Biotechnol. Spring 84-86: 201-216, 2000. PubMed: 10849789

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