| Vector Information |
Construct size (kb): 21.00
Vector: pMS2 (plasmid)
Construction: pBR322; pMS1
Marker(s): ampR, kanR
Replicon: pMB1, pMS1
Cloning sites: BamHI, EcoRI
Possible host(s): E. coli, Corynebacterium (ATCC® 13059™), Rhodococcus equi |
| Comments |
Ampicillin and kanamycin resistance are expressed in E. coli; kanamycin resistance is expressed in Rhodococcus equi.
-U.S. Patent No. 4,920,054 dated Apr. 24, 1990
Restriction digests of the clone give the following sizes (kb): BamHI--21.0; EcoRI--21.0; HindIII--6.8 (doublet), 3.6, 1.45, 1.1.
-ATCC staff
pBR322 cut with BamHI, and pMS1 (a 19 kb plasmid from Rhodococcus equi) partially digested with Sau3AI, were ligated to form pMS2. The construct retains ampR and ori from pBR322 and kanR and ori from pMS1.
-U.S. Patent No. 4,920,054 dated Apr. 24, 1990
A shuttle vector stably maintained in E. coli as well as members of the coryneform group, namely Corynebacterium (ATCC® 13059™) and Rhodococcus equi.
-U.S. Patent No. 4,920,054 dated Apr. 24, 1990 |
