| 產品名稱 |
pADA5-29 |
| 商品貨號 |
B176304 |
| Designations |
pADA5-29 |
| GenBank Number |
M10319
|
| Species |
Mus musculus, mouse |
| Depositors |
V Ramamurthy |
| Vector |
Construct size (kb): 5.699999809265137 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.399999976158142 Tissue: fibroblast B-1/50 cell line Gene product: adenosine deaminase [Ada] |
| Insert Size (kb) |
1.400 |
| Media |
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
The insert contains restriction sites at the following positions: BglI--105; PstI--227; PstI--413; BamHI--815; HindIII--996. Restriction digests of the clone give the following sizes (kb): PstI--4.4, 1.0, 0.32, 0.19; EcoRI--5.7; HindIII--4.0, 1.9; AvaI--5.7; BamHI--3.8, 2.1. Contains the complete coding sequence, extending from nt -70 to nt +1330, where nt 1 is the A of the initiation codon. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Yeung CY, et al. Identification of functional murine adenosine deaminase cDNA clones by complementation in Escherichia coli. J. Biol. Chem. 260: 10299-10307, 1985. PubMed: 2410423
|
