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Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 5.8039999008178710

DESCRIPTION OF VECTOR:
Intact vector size: 5.804
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS416
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
replicon: CEN6/ARSH4, ->
marker(s): URA3, ->
terminator: CYC1, ->
promoter for expression: GPD, <-
marker(s): ampR, <-

Vector: p416 GPD (plasmid)

Promoters: Promoter for expression GPD

Construction: pRS416

Marker(s):URA3,ampR

Construct size (kb): 5.803999900817871

Features: marker(s): URA3
marker(s): ampR
promoter for expression: GPD
replicon: CEN6/ARSH4
terminator: CYC1

YX-type (expression) shuttle vector

vector with low copy number

Restriction digests of the clone give the following sizes (kb): SstI/XbaI--5.13, 0.68; EcoRI--5.8, BamHI--5.8.

Low copy number shuttle expression vector.

Temperature: 37.0°C

component of:ATCC 87669