| Vector Information |
Size (kb): 5.5999999046325680 Vector: pMB (plasmid) Promoters: Promoter MMTV LTR Construction: pM (ATCC 77383) Marker(s):ampR Construct size (kb): 5.599999904632568 Features: insert detection: lacZ'
insert detection: lacZ'amb5
marker(s): ampR
promoter: MMTV LTR
replicon: pMB1
MCS: EcoRI...HindIII
MCS: NruI...HindIII
restriction site: ClaI
restriction site: NotI
restriction site: NsiI
terminator: SV40
enhancer: RSV |
| Comments |
Restriction digests of the clone give the following sizes (kb): PvuI--2.5, 1.8, 1.3; EcoRI--3.0, 2.6; HindIII--2.6, 1.7, 1.3; BglII--5.6; ClaI--4.3, 1.3. The plasmid contains the following restriction sites (bp from position 0); NotI--0; EcoRI--9, 3071; ClaI--425, 1560, 1745; Drd--1398, 3583, 5452; PvuI--1596, 2953, 4741; BglII--1782; SphI--2907, 3110; NsiI--2909; HindIII--99, 1794, 3112; BsaI--4441. Expression vector containing two lacZalpha-based multiple cloning sites, with the second containing an amber mutation and being flanked by an RSV enhancer-MMTVLTR promoter at one end and splicing and polyadenylation signals at the other. The first site was designed for insertion of a marker selectable in mammalian cells, and the second for the sequence to be expressed. Both sites permit detection of inserts by alpha complementation, with discrimination between the two provided by the amber mutation in the second lacZ. |
| References |
Wang Q, et al. Mammalian expression vectors with modulatable promoters and two multiple cloning sites. Gene 119: 155-161, 1992. PubMed: 1327962
J J McCormick, personal communication
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