| 產品名稱 |
pRY255 |
| 商品貨號 |
B176656 |
| Designations |
pRY255 |
| Depositors |
RR Yocum |
| U.S. Patent |
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level |
1 |
| Host |
Distribution host: Escherichia coli YMC9 |
| Vector Information |
Size (kb): 17.8999996185302700 Vector: pRY255 (plasmid) Promoters: Promoter CYC1 Construction: pRY253 Marker(s):ampR G418R Construct size (kb): 17.89999961853027 Features: marker(s): G418R
marker(s): ampR
other: HO
promoter: CYC1
replicon: pMB1
coding sequence: GAL1/lacZ fusion |
| Applications |
YI-type (integrating) shuttle vector vector permitting positive selection for integration vector permitting visual detection of recombinants |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--11.0, 7.4; KpnI--19.0; SalI--19.0. A YI-type (yeast integrating, HO gene) shuttle vector that confers G418 resistance under control of the CYC1 promoter. Expresses a GAL1/lacZ fusion product allowing color selection. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Yocum RR, et al. Industrial yeast comprising an integrated glucoamylase gene. US Patent 5,422,267 dated Jun 6 1995
|
| Shipped |
freeze-dried |
| Shipping Information |
Distributed: freeze-dried |
