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Size (kb): 9.3000001907348630

Vector: pUFR027 (plasmid)

Promoters: Promoter lac

Construction: pSa747, pUCD550, pUC4Kan, pLAFR3, pUC18

Marker(s):neoR

Construct size (kb): 9.300000190734863

Features: insert detection: lacZ'
marker(s): neoR
promoter: lac
replicon: oriW

shuttle vector

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): EcoRI--9.4; PstI--6.8, 1.4, 1.25; SmaI--7.7, 1.9.

Uses interruption of lacZalpha.

Shuttle vector permitting visual detection of recombinants.

The partition locus from Agrobacterium plasmid pTAR stabilizes the plasmid in X. citri and X. campestris pvs. campestris, vesicatoria, malvacearum, and translucens grown in broth culture.

The order of the major features in this plasmid is: lacZalpha with MCS (pUC18) - oriW (pSa747, IncW) - parA+ (pUCD550) - aphA (pUC4Kan) - mob(P) (pLAFR3, Mob+).

Temperature: 37.0°C