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<em>Saccharomyces cerevisiae</em> Meyen ex E.C. Hansen
<em>Saccharomyces cerevisiae</em> Meyen ex E.C. Hansen
規格:
貨期:
編號:B176670
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Saccharomyces cerevisiae Meyen ex E.C. Hansen
商品貨號 B176670
Deposited As Saccharomyces cerevisiae Hansen, teleomorph
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Strain Designations NRC 5140 [ATCC 66527, C468, CMCC 1398, LL20, NCYC 1445]
Alternate State Candida robusta Diddens et Lodder
Application
transformation host
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Genotype MATalpha leu2-3 leu2-112 his3-11 his3-15 [psi+] [cir+]
Preceptrol&reg; no
Mating Type alpha
Ploidy haploid
Comments
Expression of Aspergillus awamori glucoamylase gene
Sensitive to Kluyveromyces lactis toxin
Medium ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 25.0°C
Name of Depositor RK Latta
References

Suominen I, et al. Enhanced recovery and purification of Aspergillus glucoamylase from Saccharomyces cerevisiae by the addition of poly(aspartic acid) tails. Enzyme Microb. Technol. 15: 593-600, 1993. PubMed: 7763957

Flory N, et al. Thermosensitive mutants of Aspergillus awamori glucoamylase by random mutagenesis: inactivation kinetics and structural interpretation. Protein Eng. 7: 1005-1012, 1994. PubMed: 7809026

Butler AR, et al. Analysis of the response of Saccharomyces cerevisiae cells to Kluyveromyces lactis toxin. J. Gen. Microbiol. 137: 1749-1757, 1991. PubMed: 1955863

Erratt JA, Nasim A. Cloning and expression of a Saccharomyces diastaticus glucoamylase gene in Saccharomyces cerevisiae and Schizosaccharomyuces pombe. J. Bacteriol. 166: 484-490, 1986. PubMed: 3009402

McNeil JB, et al. High frequency recombination and the expression of genes cloned on chimeric yeast plasmids: identification of a fragment of 2-um circle essential for transformation. Curr. Genet. 2: 17-25, 1980.

Fierobe HP, et al. Mutational modulation of substrate bond-type specificity and thermostability of glucoamylase from Aspergillus awamori by replacement with short homologue active site sequences and thiol/disulfide engineering. Biochemistry 35: 8696-8704, 1996. PubMed: 8679632

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李經理 13626845108 972239479
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