| 產品名稱 |
pMTD |
| 商品貨號 |
B176679 |
| Designations |
pMTD |
| GenBank Number |
U21960
|
| Species |
Mus musculus, mouse |
| Depositors |
VM Morales, RS Blumberg |
| Vector |
Construct size (kb): 4.400000095367432 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.375 Tissue: thymus, female, 6-8 weeks Gene product: calcium modulating ligand( calcium-signal modulating cyclophilin ligand, calcium-modulating cyclophilin ligand) [Caml] |
| Insert Size (kb) |
1.375 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.4; EcoRI/HincII--3.0, 1.1, 0.3; HincII--4.4; PstI--3.4, 1.0. Insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.46; HincII--1.10; NcoI--0.81; PstI--0.97; PvuII--0.35, 0.53, 0.76; SacI--0.42; SmaI--0.18. Bacteria containing this plasmid grow slowly and yield less DNA than other pBluescript clones. Clones should be grown using low levels of ampicillin (50 mcg/ml) and a single colony should be isolated, avoiding those colonies that grow quickly. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Kim HS, et al. Cloning of the gene encoding the mouse homologue of the human calcium signal-modulating ligand. Gene 163: 323-324, 1995. PubMed: 7590290
Victor M Morales, personal communication
|
