| Applications |
It reacts with a single protein in Western blots of A-431 cells (ATCC CRL-1555) corresponding to purified 130 kDa myocardial calpastatin. The antibody is specific for human calpastatin. The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting calpastatin. |
| Comments |
Animals were immunized with calpastatin isolated from human erythrocytes. The antibody is specific for human calpastatin. It reacts with a single protein in Western blots of A-431 cells (ATCC CRL-1555) corresponding to purified 130 kDa myocardial calpastatin. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting calpastatin. |
| References |
Lane RD, Mellgren RL. A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells. Exp. Cell Res. 203: 5-16, 1992. PubMed: 1426051
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
|
