| Insert |
DNA: cDNA Insert lengths(kb): 2.880000114440918 Tissue: placenta Gene product: collagen, type IV, alpha 1 [COL4A1] Alleles: A2, B1, B2, C2, D1, D2, A1, A3, C1 |
| Comments |
The insert contains an EcoRI site at nt 3467 of the published sequence. Restriction digests of the clone give the following sizes (kb): XhoI-->23.0, 15.0; KpnI-->23.0, 20.0, 1.6, 1.3; BamHI--17.0, 12.0, 7.0 (doublet), 5.6; SmaI--21.0, 11.5, 8.8, 6.6; EcoRI--24.0, 20.0, 1.7, 1.2; This insert contains sequences encoding part of the triple-helical domain and the non-collagenous domain. HP-2 overlaps HP-11 (ATCC 65034, 65035) on the 5' end and overlaps HT-21 (ATCC 65036, 65037) on the 3' end. |