ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 1.1; HpaI--5.6; XhoI--5.6; EcoRI--4.3, 1.0; EcoRI/HpaI--4.5, 1.1; XhoI/HindIII--4.7, 0.5.
Insert was subcloned from pcD(SRalpha)-22-1 into pBR322. The insert is in a reverse orientation.
References
Otsuka T, et al. Isolation and characterization of an expressible cDNA encoding human IL-3. Induction of IL-3 mRNA in human T cell clones. J. Immunol. 140: 2288-2295, 1988. PubMed: 3127463
